Adding DNA with a recombinase
The scissors - a combination of gene-gripping "zinc fingers" and a DNA-cutting enzyme called a nuclease - find the target sequence, latch on to it and cut it.
Targetting DNA with molecular scissors
Description
B
Natural
enzymes fix the break,
but in the process sometimes chop out the broken section and replace it
with a new chunk of DNA - cunningly added by engineers - in a poorly
understood process called homologous recombination.
Viral enzymes called recombinases cut DNA at target site 1 and target site 2, and then join the ends together. So if you create a circular piece of DNA containing target site 1, recombinases will integrate this DNA into the genome of any cell containing target site 2
3
